首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子Ⅱ型胶原C端肽(CTX-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTX-Ⅱ含量。CTX-Ⅱ是一种由Ⅱ型胶原降解产生的肽段，是骨关节炎（OA）的潜在生物标志物。其水平与关节损伤密切相关，可用于OA的早期诊断和病情评估。研究显示，CTX-Ⅱ在OA患者关节液中水平升高，可作为疾病进展及预后的指标。试剂盒检测范围为0.625 ng/mL-40 ng/mL，常用于骨关节炎、类风湿性关节炎等疾病模型中软骨退行性病变的机制研究。适用于科研场景中评估实验动物软骨代谢状态、筛选保护软骨组织的候选化合物，或探究病理条件下胶原降解的动态变化，可为体外实验提供稳定的数据支持，助力关节疾病相关的基础研究与药物开发。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cross linked C-telopeptide of type 2 collagen (CTX-2),_x000D_ cross linked C-telopeptide of type II collagen (CTX-II)
缩写：CTX-Ⅱ
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of rabbit CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-103 | ||||
| EDTA plasma (n=4) | 95 | 89-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5472.6522.6002.462 202.0371.9622.0001.862 101.4491.3761.4131.275 50.9020.8660.8840.746 2.50.5140.5260.5200.382 1.250.3320.3470.3400.202 0.6250.2460.2530.2500.112 00.1390.1360.138 | ||||||
货期：3-5 working days
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