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产品描述:人Ⅱ型胶原交联C端肽(CTX-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、尿液样本中的CTX-含量。靶点研究是药物研发的关键,涉及识别疾病相关分子或细胞结构,通过药物相互作用产生治疗效果。研究机制包括基因组学、蛋白质组学、生物信息学和高通量筛选技术,旨在发现新靶点,设计精准药物。CTX-320是一种基于CRISPR/Cas9技术的基因编辑药物,用于治疗代谢病、动脉粥样硬化和主动脉瓣狭窄。试剂盒检测范围为0.312 ng/mL-20 ng/mL,常用于研究关节代谢动态及软骨损伤机制;适用于体外研究关节疾病模型中的生物标志物表达、药物干预效果评估或病理机制探索;为软骨代谢研究及骨关节相关疾病的基础研究提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Cross Linked C-Telopeptide Of Type 2 Collagen ,CTX-2,_x000D_ Cross Linked C-Telopeptide Of Type II Collagen ,CTX-II
缩写:CTX-
种属:Homo sapiens (Human)
样本类型:serum, plasma, urine
检测范围:0.312 ng/mL-20 ng/mL
灵敏度:0.078 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 93 | ||||
Range % | 86-98 | |||||
1:2 | Average % | 100 | ||||
Range % | 96-104 | |||||
1:4 | Average % | 99 | ||||
Range % | 92-105 | |||||
1:8 | Average % | 92 | ||||
Range % | 87-98 |
回收率:
The recovery of human CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 90-100 | ||||
EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected 202.4622.6712.5672.423 101.9591.9641.9621.818 51.3981.3451.3721.228 2.50.8220.8370.8300.686 1.250.5460.5580.5520.408 0.6250.3820.3960.3890.245 0.3120.2810.2970.2890.145 00.1420.1460.144 |
货期:3-5 working days
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