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Mouse cross linked C-telopeptide of type I collagen (CTX-Ⅰ) ELISA Kit

  • 中文名： 小鼠Ⅰ型胶原C端肽(CTX-Ⅰ)ELISA Kit
货号: IPD-E12782m
Price： ￥2500
数量：
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  • 产品描述：
    小鼠Ⅰ型胶原C端肽(CTX-Ⅰ)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的CTX-Ⅰ含量。CTX-Ⅰ，即Ⅰ型胶原C端肽，是胶原蛋白分解代谢的关键产物，其浓度变化反映骨基质降解活性，常用于骨代谢异常、骨质疏松模型研究及骨相关疾病的机制探索。研究机制涉及CTX-Ⅰ在骨吸收过程中的生成和作用，以及与骨代谢相关疾病的关系。试剂盒检测范围为0.23 ng/ml-15 ng/ml.，CTX - Ⅰ是Ⅰ型胶原蛋白分解代谢的关键产物，其浓度变化可反映骨基质降解活性，常用于骨代谢异常、骨质疏松模型研究及骨相关疾病的机制探索。科研场景中可用于评估骨吸收标志物水平，例如在骨质疏松动物模型中动态监测骨质流失程度，或结合药物干预实验探究骨代谢调控机制。支持科研人员高效完成骨代谢相关的基础研究与药效评价。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    cross linked C-telopeptide of type 1 collagen (CTX-1),_x000D_cross linked C-telopeptide of type I collagen (CTX-I)
  • 缩写：
    CTX-Ⅰ
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.23 ng/ml-15 ng/ml.
  • 灵敏度：
    0.12 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    84


    Range %

    80-92


    1:2

    Average %

    87


    Range %

    81-94


    1:4

    Average %

    88


    Range %

    82-95


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of mouse CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    85

    81-91


    EDTA plasma (n=4)

    87

    82-93


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average




    15

    0.195

    0.204

    0.200




    7.5

    0.246

    0.260

    0.253




    3.75

    0.339

    0.348

    0.344




    1.87

    0.518

    0.529

    0.524




    0.94

    0.851

    0.841

    0.846




    0.47

    1.272

    1.253

    1.262




    0.23

    1.838

    1.784

    1.811




    0

    2.512

    2.525

    2.518





  • 货期：
    3-5 working days

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