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产品描述：人溶酶体保护蛋白(CTSA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTSA含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中探索CTSA在疾病模型中的表达变化、药物干预对溶酶体功能的影响，或通过体液/组织样本评估生物标志物水平；可为细胞生物学、病理机制研究及药物开发提供可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta galactosidase 2 ELISA Kit; BETA GALACTOSIDASE PROTECTIVE PROTEIN ELISA Kit; beta-galactosidase 2 ELISA Kit; beta-galactosidase protective protein ELISA Kit; betagalactosidase 2 ELISA Kit; Carboxypeptidase C ELISA Kit; Carboxypeptidase L ELISA Kit; carboxypeptidase Y-like kininase ELISA Kit; Cathepsin A ELISA Kit; Ctsa ELISA Kit; deamidase ELISA Kit; EC 3.4.16.5 ELISA Kit; Glactosialidosis ELISA Kit; GLB2 ELISA Kit; Goldberg Syndrome ELISA Kit; GSL ELISA Kit; lysosomal carboxypeptidase A ELISA Kit; Lysosomal protective protein 20 kDa chain ELISA Kit; Lysosomal protective protein ELISA Kit; Lysosomal protective protein deficiency ELISA Kit; NEURAMINIDASE BETA GALACTOSIDASE EXPRESSION; NGBE ELISA Kit; Neuraminidase deficiency with beta-galactosidase deficiency ELISA Kit; NGBE ELISA Kit; OTTHUMP00000031778 ELISA Kit; OTTHUMP00000031781 ELISA Kit; PPCA ELISA Kit; PPCA deficiency ELISA Kit; PPGB ELISA Kit; PPGB_HUMAN ELISA Kit; Protective protein cathepsin A ELISA Kit; Protective protein for beta galactosidase ELISA Kit; Protective protein for beta-galactosidase ELISA Kit; Protective protein/cathepsin A deficiency ELISA Kit; urinary kininase ELISA Kit
缩写：CTSA
Uniprot No.：P10619
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:4 | Average % | 106 | ||||
| Range % | 102-109 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human CTSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7452.8492.7972.6935002.2572.4122.3352.2312501.6161.7171.6671.5631250.9430.9650.9540.85062.50.5470.5240.5360.43231.20.3540.3630.3590.25515.60.2450.2540.2500.14600.1030.1050.104 | ||||||
货期：3-5 working days
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