首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β-连环蛋白(CTNNB1/CTNNB/OK/SW-cl.35/PRO2286)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的CTNNB1含量。CTNNB1 编码 β-连环蛋白，在细胞黏附和 Wnt 信号通路中至关重要。其异常激活与肿瘤发生发展、侵袭转移密切相关。研究中，常聚焦其在信号传导机制，以及与其他蛋白相互作用，以探索开发针对 CTNNB1 的肿瘤治疗新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中探索癌症生物学机制、信号转导调控研究、细胞间相互作用分析等领域，特别为结直肠癌、肝癌等肿瘤模型研究及药物干预实验提供可靠的蛋白表达水平检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：b-catenin ELISA Kit; Beta catenin ELISA Kit; Beta-catenin ELISA Kit; Cadherin associated protein ELISA Kit; Catenin (cadherin associated protein); beta 1; 88kDa ELISA Kit; Catenin beta 1 ELISA Kit; Catenin beta-1 ELISA Kit; CATNB ELISA Kit; CHBCAT ELISA Kit; CTNB1_HUMAN ELISA Kit; CTNNB ELISA Kit; CTNNB1 ELISA Kit; DKFZp686D02253 ELISA Kit; FLJ25606 ELISA Kit; FLJ37923 ELISA Kit; OTTHUMP00000162082 ELISA Kit; OTTHUMP00000165222 ELISA Kit; OTTHUMP00000165223 ELISA Kit; OTTHUMP00000209288 ELISA Kit; OTTHUMP00000209289 ELISA Kit
缩写：CTNNB1
Uniprot No.：P35222
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTNNB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of human CTNNB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-104 | ||||
| EDTA plasma (n=4) | 89 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.1992.2772.2382.0755001.7431.6631.7031.5402501.1791.1571.1681.0051250.7940.8000.7970.63462.50.4720.4600.4660.30331.20.3270.3330.3300.16715.60.2320.2260.2290.06600.1630.1620.163 | ||||||
货期：3-5 working days
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