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Human Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

  • 中文名： 人粒细胞集落刺激因子(G-CSF)ELISA Kit
货号: IPD-E04563h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人粒细胞集落刺激因子(G-CSF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CSF3含量。CSF3，即集落刺激因子3，是一种由多种细胞产生的细胞因子，主要在骨髓造血细胞中表达。其作用机制涉及与特异性受体结合，激活JAK/STAT、MAPK等信号通路，促进粒细胞增殖、分化和存活，参与免疫系统发育和功能，尤其对中性粒细胞生成和活化起重要作用。试剂盒检测范围为7.8 pg/ml-500 pg/ml，灵敏度为1.95 pg/ml。适用于体外实验研究，如探究炎症反应机制、评估药物对造血系统的影响、构建中性粒细胞减少症动物模型等基础科研场景，为免疫学、血液学及肿瘤治疗相关研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C17orf33 ELISA Kit; Colony stimulating factor 3 (granulocyte) ELISA Kit; Colony stimulating factor 3 ELISA Kit; CSF 3 ELISA Kit; CSF beta ELISA Kit; CSF3 ELISA Kit; CSF3_HUMAN ELISA Kit; CSF3OS ELISA Kit; Csfg ELISA Kit; Filgrastim ELISA Kit; G-CSF ELISA Kit; GCSA ELISA Kit; GCSF ELISA Kit; Granulocyte colony stimulating factor ELISA Kit; Granulocyte colony-stimulating factor ELISA Kit; Lenograstim ELISA Kit; Macrophage granulocyte inducer 2 ELISA Kit; MGC45931 ELISA Kit; MGI 2 ELISA Kit; Pluripoietin ELISA Kit
  • 缩写：
    CSF3
  • Uniprot No.：
    P09919
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/ml -500 pg/ml
  • 灵敏度：
    1.95 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    86-102


    1:2

    Average %

    97


    Range %

    91-108


    1:4

    Average %

    93


    Range %

    88-100


    1:8

    Average %

    95


    Range %

    86-98


  • 回收率：

    The recovery of human G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    89-98


    EDTA plasma (n=4)

    87

    84-92


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    500

    2.414

    2.503

    2.459

    2.332


    250

    1.552

    1.467

    1.509

    1.383


    125

    0.915

    0.973

    0.944

    0.817


    62.5

    0.616

    0.629

    0.622

    0.496


    31.2

    0.313

    0.331

    0.322

    0.195


    15.6

    0.216

    0.225

    0.221

    0.094


    7.8

    0.171

    0.180

    0.176

    0.049


    0

    0.129

    0.125

    0.127




  • 货期：
    3-5 working days

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