首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人软骨酸性蛋白1(CRTAC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CRTAC1含量。试剂盒检测范围为4.68 ng/mL-300 ng/mL，该产品适用于软骨相关疾病机制研究、生物标志物筛选及药物干预效果评估等科研领域，尤其适合在血清、血浆等复杂基质中分析CRTAC1的动态变化，为软骨代谢研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：68 kDa chondrocyte expressed protein ELISA Kit; 68 kDa chondrocyte-expressed protein ELISA Kit; Acidic secreted protein in cartilage ELISA Kit; ASPIC ELISA Kit; ASPIC1 ELISA Kit; Cartilage acidic protein 1 ELISA Kit; Cartilage acidic protein 1 precursor ELISA Kit; CEP 68 ELISA Kit; CEP-68 ELISA Kit; CEP68 ELISA Kit; Chondrocyte expressed protein 68 kDa CEP 68 ELISA Kit; CRAC1_HUMAN ELISA Kit; CRTAC1 ELISA Kit; Protein CRTAC1-B ELISA Kit; W307 ELISA Kit
缩写：CRTAC1
Uniprot No.：Q9NQ79
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：4.68 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CRTAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 91 | ||||
| Range % | 87-96 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:20 | Average % | 102 | ||||
| Range % | 99-103 | |||||
| 1:40 | Average % | 88 | ||||
| Range % | 86-91 | |||||
回收率：
| The recovery of human CRTAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-93 | ||||
| EDTA plasma (n=4) | 102 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3002.5222.7352.6292.4441502.0432.1022.0731.888751.3171.3151.3161.13137.50.8750.8570.8660.68118.750.4700.4450.4580.2739.370.3660.3800.3730.1884.680.2540.2520.2530.06800.1860.1840.185 | ||||||
货期：3-5 working days
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