首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子C反应蛋白(CRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CRP含量。CRP即C反应蛋白，是一种急性时相反应蛋白。其背景在于机体受感染或组织损伤时，血浆中浓度急剧升高。研究机制方面，它可激活补体系统、调节淋巴细胞功能等，在炎症反应、免疫调节等过程中发挥重要作用，是临床常用的炎症指标。试剂盒检测范围为7.8 pg/mL-2000 pg/mL，动物模型中的免疫调控机制研究、炎症相关疾病模型评估、药物干预效果分析、实验性关节炎模型、感染性疾病模型或代谢综合征研究中血清/血浆 CRP 的动态监测、探索炎症通路机制及潜在治疗靶点本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CRP ELISA kit; PTX1C-reactive protein ELISA kit
缩写：CRP
Uniprot No.：P02742
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：7.8 pg/mL-2000 pg/mL
灵敏度：2 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2000 | Average % | 91 | ||||
| Range % | 82-96 | |||||
| 1:4000 | Average % | 90 | ||||
| Range % | 85-98 | |||||
| 1:8000 | Average % | 94 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of rabbit CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-104 | ||||
| EDTA plasma (n=4) | 91 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.1960.1980.197 5000.3530.3710.362 1250.6690.6510.660 31.31.0841.0351.060 7.82.0181.9741.996 02.9133.1223.018 | ||||||
货期：3-5 working days
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