首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人氨甲酰磷酸合成酶 [氨],线粒体(CPS1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPS1含量。CPS1（氨基甲酰磷酸合成酶1）是尿素循环中的关键限速酶，参与氨的解毒过程。研究发现，CPS1与多种恶性肿瘤如肝癌、肺癌、结直肠癌等密切相关，通过影响细胞增殖、凋亡等生物学行为，影响肿瘤的发生发展及患者预后。研究CPS1在肿瘤中的作用机制，有助于开发新的治疗策略。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于科研领域探究肝脏代谢功能、遗传性代谢疾病模型研究、药物干预对尿素循环的影响等实验需求；为研究者评估生物体内氨代谢水平、探索肝病发生机制及代谢调控通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Carbamoyl phosphate synthase [ammonia] ELISA Kit; Carbamoyl phosphate synthase [ammonia] mitochondrial ELISA Kit; Carbamoyl phosphate synthase ELISA Kit; Carbamoyl phosphate synthetase 1 ELISA Kit; Carbamoyl phosphate synthetase 1 mitochondrial ELISA Kit; Carbamoyl phosphate synthetase I ELISA Kit; Carbamoyl-phosphate synthase [ammonia] ELISA Kit; Carbamoyl-phosphate synthetase I ELISA Kit; Carbamoylphosphate synthase ELISA Kit; Carbamoylphosphate synthetase 1 ELISA Kit; Carbamoylphosphate synthetase I ELISA Kit; CPS 1 ELISA Kit; Cps1 ELISA Kit; CPSase 1 ELISA Kit; CPSase I ELISA Kit; CPSASE1 ELISA Kit; CPSM_HUMAN ELISA Kit; mitochondrial ELISA Kit; MS738 ELISA Kit
缩写：CPS1
Uniprot No.：P31327
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CPS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 93-107 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of human CPS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.3062.3672.3372.191 25002.1712.1912.1812.035 12501.8071.8471.8271.681 6251.3931.3711.3821.236 312.50.8010.8240.8130.667 1560.5520.5910.5720.426 780.3620.3710.3670.221 00.1430.1490.146 | ||||||
货期：3-5 working days
×
