首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅲ型胶原(Col Ⅲ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅲ含量。Col Ⅲ胶原蛋白是细胞外基质的重要组成部分，参与组织结构和功能。其研究背景涉及胶原蛋白在组织修复、纤维化疾病中的作用。研究机制主要围绕Col Ⅲ的合成、降解及其与信号通路的关系，旨在揭示其在生理和病理过程中的作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于实验室对大鼠纤维化疾病（如肝纤维化、肺纤维化）的机制研究、药物干预效果评估或组织工程中胶原代谢的动态监测，满足科研场景中高通量、快速检测的需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：Col Ⅲ
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-94 | |||||
回收率：
| The recovery of rat Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.5852.5322.5592.415 51.7951.7191.7571.613 2.51.0831.0211.0520.908 1.250.6570.7050.6810.537 0.6250.4180.4500.4340.290 0.3120.2990.3140.3070.163 0.1560.2420.2290.2360.092 00.1460.1410.144 | ||||||
货期：3-5 working days
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