首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Ⅱ型胶原(Col Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅱ含量。Col Ⅱ，即II型胶原蛋白，是构成透明软骨的基础，由胶原蛋白II型α1链的同源三聚体组成。它在软骨中占比高达50%，负责提供组织的拉伸强度和蛋白聚糖的捕获。在研究中，Col Ⅱ的合成和分泌在体外培养的软骨细胞中持续存在，但其合成在细胞快速增殖时会被关闭，这为组织工程软骨提供了新的策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外科研实验中定量分析Col Ⅱ的表达动态，例如研究软骨组织工程材料的生物相容性、关节炎模型中基质代谢变化、软骨细胞分化调控机制等。为软骨相关疾病机制探索、药物干预效果评估等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
缩写：Col Ⅱ
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:400 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.4782.3872.4332.341 10001.8111.7521.7821.690 5001.2371.1421.1901.098 2500.8630.8130.8380.746 1250.3980.3740.3860.294 62.50.2110.1980.2050.113 31.250.1450.1410.1430.051 00.0930.0900.092 | ||||||
货期：3-5 working days
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