首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅰ含量。Col Ⅰ即Ⅰ型胶原蛋白，是细胞外基质的主要成分。其背景在于它广泛存在于多种组织，对维持组织正常结构和功能起重要作用。研究机制方面，围绕其在组织纤维化、肿瘤微环境等过程中的合成、降解及信号传导途径展开，旨在寻找相关疾病的治疗靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于体外实验环境下对大鼠组织修复机制、纤维化疾病模型评估或骨骼代谢相关研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
缩写：Col Ⅰ
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of rat Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 98 | 93-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.7012.7622.7322.537 25001.9031.9541.9291.734 12501.1951.2071.2011.006 6250.8020.7430.7730.578 3120.4890.4660.4780.283 1560.3760.3590.3680.173 780.2580.2430.2510.056 00.2010.1890.195 | ||||||
货期：3-5 working days
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