首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆样本中的Col Ⅰ含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于纤维化疾病模型研究、骨代谢机制探索以及再生医学中胶原沉积的动态分析。该产品兼容多种实验场景，包括体外细胞培养体系中胶原分泌的定量评估、动物模型组织修复过程的生物标志物追踪，以及临床前研究中Col Ⅰ代谢相关病理生理机制的解析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 1,Col 1,_x000D_Collagen Type I,Col I
缩写：Col Ⅰ
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 91-110 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.9212.9852.9532.767 502.3562.3782.3672.181 251.8261.8751.8511.665 12.51.2211.2561.2391.053 6.250.7960.8000.7980.612 3.120.5120.5460.5290.343 1.560.3020.3260.3140.128 00.1880.1840.186 | ||||||
货期：3-5 working days
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