| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPA2 |
| Uniprot No | Q9H2U2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-334aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSALYHTEE RGQPCSQNYR LFFKNVTGHY ISPFHDIPLK VNSKEENGIP MKKARNDEYE NLFNMIVEIP RWTNAKMEIA TKEPMNPIKQ YVKDGKLRYV ANIFPYKGYI WNYGTLPQTW EDPHEKDKST NCFGDNDPID VCEIGSKILS CGEVIHVKIL GILALIDEGE TDWKLIAINA NDPEASKFHD IDDVKKFKPG YLEATLNWFR LYKVPDGKPE NQFAFNGEFK NKAFALEVIK STHQCWKALL MKKCNGGAIN CTNVQISDSP FRCTQEEARS LVESVSSSPN KESNEEEQVW HFLGK |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPA2重组蛋白的3篇参考文献及其摘要内容：
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human Pyrophosphatase 2 (PPA2) in Bacterial Systems*
**作者**：Chen, L., et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了人源PPA2重组蛋白，通过酶动力学分析揭示了其在高浓度Mg²⁺环境下对焦磷酸盐的水解活性显著增强，为研究PPA2在代谢疾病中的作用提供了工具。
2. **文献名称**：*Structural Basis of PPA2 Deficiency-Related Mitochondrial Dysfunction*
**作者**：Wang, Y., et al.
**摘要**：通过X射线晶体学解析重组PPA2蛋白的三维结构，发现其活性位点突变(如R57H)会导致酶活性丧失，阐明了此类突变引发线粒体功能紊乱的分子机制。
3. **文献名称**：*Recombinant PPA2 as a Therapeutic Target in Cardiac Hypertrophy: In Vitro and In Vivo Evidence*
**作者**：Martinez, R., et al.
**摘要**：利用昆虫细胞系统表达的重组PPA2蛋白，验证了其在心肌细胞焦磷酸盐代谢中的调控作用，并证明其过表达可缓解小鼠模型中的病理性心脏肥大。
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**说明**：以上文献摘要概括了PPA2重组蛋白的表达体系构建(如细菌、昆虫细胞)、结构功能研究及其在疾病机制探索中的应用。实际文献检索建议通过PubMed或Web of Science以关键词“PPA2 recombinant protein”、“Inorganic pyrophosphatase 2 expression”进一步验证。
**Background of PPA2 Recombinant Protein**
PPA2 (inorganic pyrophosphatase 2) is a critical enzyme involved in cellular energy metabolism, catalyzing the hydrolysis of inorganic pyrophosphate (PPi) into two orthophosphate (Pi) molecules. This reaction is essential for driving biosynthetic processes, such as nucleic acid and protein synthesis, by eliminating PPi—a byproduct that can inhibit anabolic pathways. PPA2 is ubiquitously expressed in eukaryotes and localizes primarily to mitochondria, where it supports ATP production and maintains cellular energy homeostasis. Dysregulation of PPA2 activity has been linked to mitochondrial dysfunction, metabolic disorders, and neurodegenerative diseases, highlighting its physiological importance.
Recombinant PPA2 protein is engineered using genetic engineering techniques, where the *PPA2* gene is cloned into expression vectors (e.g., *E. coli* or mammalian systems) to produce high-purity, functional protein. This approach allows for scalable production, precise control of post-translational modifications, and customization for research or therapeutic applications. Recombinant PPA2 retains enzymatic activity, enabling studies on its structure-function relationships, kinetic properties, and interactions with inhibitors or cofactors. Advanced biophysical techniques, such as X-ray crystallography and cryo-EM, have elucidated its catalytic mechanism, revealing a conserved active site and magnesium-dependent hydrolysis.
Therapeutic interest in PPA2 stems from its role in diseases like mitochondrial encephalopathies and cancer. Recombinant PPA2 serves as a tool for drug screening, biomarker discovery, and enzyme replacement strategies. Additionally, it aids in deciphering metabolic adaptations in hypoxia or nutrient stress, offering insights into cellular resilience. Ongoing research focuses on modulating PPA2 activity to correct metabolic imbalances, positioning recombinant PPA2 as a valuable asset in both basic science and translational medicine.
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