首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人降钙素基因相关肽(CGRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CGRP含量。CGRP（降钙素基因相关肽）是一种神经肽。在它在偏头痛等疾病发病机制中扮演重要角色。研究机制上，它能扩张脑血管、调节神经炎症。针对 CGRP 及其受体开发药物以阻断其作用，成为偏头痛等治疗的新策略。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于体外研究CGRP在神经生物学、心血管功能、疼痛机制或炎症模型中的动态变化，例如评估实验动物模型中CGRP的释放水平、细胞因子刺激下培养细胞的分泌特征，或药物干预对组织内CGRP表达的影响等科研用途本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta type CGRP ELISA Kit; Beta-CGRP ELISA Kit; Beta-type CGRP ELISA Kit; CALC2 ELISA Kit; Calcb ELISA Kit; CALCB_HUMAN ELISA Kit; Calcitonin gene related peptide 2 ELISA Kit; Calcitonin gene related peptide II ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; Calcitonin related polypeptide; beta ELISA Kit; CGRP II ELISA Kit; CGRP-II ELISA Kit; CGRP2 ELISA Kit; CGRPII ELISA Kit; FLJ30166 ELISA Kit
缩写：CGRP
Uniprot No.：P10092
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.6602.5112.5862.432502.0802.1522.1161.962251.4511.3661.4091.25512.50.7950.7440.7700.6166.250.4910.5230.5070.3533.120.3150.3250.3200.1661.560.2480.2440.2460.09200.1570.1510.154 | ||||||
货期：3-5 working days
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