| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69 |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ERLIN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ERLIN1抗体的3篇参考文献示例(模拟内容，非真实文献)：
---
1. **文献名称**: "ERLIN1 regulates endoplasmic reticulum-associated degradation through interaction with VCP"
**作者**: Brown EJ, et al.
**摘要**: 本研究利用ERLIN1特异性抗体，揭示了ERLIN1蛋白通过结合VCP(p97)参与内质网相关降解(ERAD)的分子机制。Western blot和免疫共沉淀实验证明ERLIN1在泛素化蛋白清除中的关键作用。
2. **文献名称**: "ERLIN1 forms a complex with ERLIN2 and modulates cholesterol metabolism"
**作者**: Smith LM, et al.
**摘要**: 通过免疫荧光和抗体阻断实验，发现ERLIN1与ERLIN2形成复合物，调控细胞内胆固醇稳态。研究使用ERLIN1抗体定位其在内质网膜脂筏结构中的分布，并发现其缺失导致脂代谢异常。
3. **文献名称**: "ERLIN1 mutations linked to hereditary spastic paraplegia impair antibody validation in cellular models"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过CRISPR/Cas9构建ERLIN1突变细胞系，结合商业化ERLIN1抗体验证其表达变化，发现突变导致蛋白稳定性下降，并与遗传性痉挛性截瘫的病理机制相关。
---
**备注**：以上文献为模拟内容，实际引用请通过PubMed或Google Scholar检索关键词(如"ERLIN1 antibody"或"ERLIN1 function")获取真实文献。
ERLIN1 (ER lipid raft-associated 1) is a transmembrane protein localized to the endoplasmic reticulum (ER) membrane, belonging to the SPFH (stomatin/prohibitin/flotillin/HflK/C) domain-containing protein family. It plays a critical role in ER-associated degradation (ERAD), a process that targets misfolded proteins for proteasomal degradation. ERLIN1 forms a complex with ERLIN2. which facilitates the recognition and ubiquitination of specific ERAD substrates, such as activated inositol 1.4.5-trisphosphate receptors (IP3Rs). This interaction is crucial for maintaining ER homeostasis and cellular stress responses. Additionally, ERLIN1 is implicated in regulating cholesterol metabolism and apoptosis by modulating sterol-regulated degradation of HMG-CoA reductase and influencing caspase activation.
Antibodies targeting ERLIN1 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate ERLIN1's role in ERAD, lipid raft organization, and disease contexts, including cancer and neurodegenerative disorders. Dysregulation of ERLIN1 has been linked to breast cancer progression and hereditary spastic paraplegia, underscoring its biomedical relevance. Researchers also utilize these antibodies to explore tissue-specific expression patterns and post-translational modifications, contributing to a deeper understanding of ER stress pathways and potential therapeutic targets.
×
