首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD5分子样蛋白(CD5L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD5L含量。CD5L作为靶点，在多项研究中受关注。它参与体内免疫调节等多种生理过程。研究机制上可能通过与相关配体结合，调节细胞信号传导通路，影响免疫细胞活化、增殖等，有望为免疫相关疾病如炎症、肿瘤等的治疗提供新思路。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于基础科研中探究CD5L在免疫代谢疾病模型（如小鼠或细胞实验）中的动态表达、评估药物干预效果或解析炎症相关分子机制等研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AAC-11 ELISA Kit; AIM ELISA Kit; API6 ELISA Kit; APOPTOSIS INHIBITOR 6 ELISA Kit; APOPTOSIS INHIBITOR OF MACROPHAGES ELISA Kit; CD5 antigen like (scavenger receptor cysteine rich family) ELISA Kit; CD5 antigen-like ELISA Kit; Cd5l ELISA Kit; CD5L_HUMAN ELISA Kit; CT 2 ELISA Kit; CT-2 ELISA Kit; Highly similar to ANTIGEN WC1.1 [Bos taurus] ELISA Kit; IgM associated peptide ELISA Kit; IgM-associated peptide ELISA Kit; Pdp ELISA Kit; PRO229 ELISA Kit; SCAVENGER RECEPTOR CYSTEINE-RICH FAMILY ELISA Kit; SP ALPHA ELISA Kit; SP-alpha ELISA Kit; Spalpha ELISA Kit
缩写：CD5L
Uniprot No.：O43866
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD5L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 96-107 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-94 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 97-104 | |||||
回收率：
| The recovery of human CD5L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-101 | ||||
| EDTA plasma (n=4) | 93 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.3352.2452.2902.1352001.4741.5771.5261.3711001.0170.9750.9960.841500.5910.6020.5970.442250.3860.3970.3920.23712.50.2780.2850.2820.1276.250.2210.2250.2230.06800.1540.1560.155 | ||||||
货期：3-5 working days
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