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产品描述：人巨噬细胞炎性蛋白3α(MIP-3α/CCL20)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的CCL20含量。CCL20是重要靶点，它属于趋化因子，在免疫调节等生理过程中发挥关键作用。研究发现其与炎症反应、肿瘤进展等密切相关，机制上主要通过与受体结合，引导免疫细胞迁移，影响细胞间信号传导，为相关疾病治疗研究提供新思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。本产品适用于体外研究MIP-3α/CCL20在炎症性疾病模型中的表达动态、免疫细胞迁移实验的机制分析，或药物干预实验中的生物标志物检测等科研用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta chemokine exodus 1 ELISA Kit; Beta-chemokine exodus-1 ELISA Kit; C C motif chemokine ligand 20 ELISA Kit; C-C motif chemokine 20 ELISA Kit; CC chemokine LARC ELISA Kit; Ccl20 ELISA Kit; CCL20(2-70) ELISA Kit; CCL20_HUMAN ELISA Kit; Chemokine (C C motif) ligand 20 ELISA Kit; Chemokine C-C motif chemokine 20 ELISA Kit; Chemokine CC motif ligand 20 ELISA Kit; CKb4 ELISA Kit; Exodus 1 ELISA Kit; Exodus ELISA Kit; LARC ELISA Kit; Liver and activation regulated chemokine ELISA Kit; Liver and activation-regulated chemokine ELISA Kit; Macrophage inflammatory protein 3 alpha ELISA Kit; MIP 3 alpha ELISA Kit; MIP 3A ELISA Kit; MIP-3-alpha ELISA Kit; MIP-3a ELISA Kit; MIP3 alpha ELISA Kit; MIP3A ELISA Kit; SCYA20 ELISA Kit; Small inducible cytokine A20 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 20 ELISA Kit; Small-inducible cytokine A20 ELISA Kit; ST38 ELISA Kit
缩写：CCL20
Uniprot No.：P78556
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-3α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 87-101 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 96-104 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 92-99 | |||||
回收率：
| The recovery of human MIP-3α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-99 | ||||
| EDTA plasma (n=4) | 96 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.0922.1002.0961.9945001.6651.7651.7151.6132501.2691.2881.2791.1771250.7750.8250.8000.69862.50.4180.3990.4090.30731.20.3240.3040.3140.21215.60.2150.2010.2080.10600.1060.0980.102 | ||||||
货期：3-5 working days
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