| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PFDN1 |
| Uniprot No | O60925 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 14-122aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MTELQAKVID TQQKVKLADI QIEQLNRTKK HAHLTDTEIM TLVDETNMYE GVGRMFILQS KEAIHSQLLE KQKIAEEKIK ELEQKKSYLE RSVKEAEDNI REMLMARRAQ |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PFDN1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Prefoldin subunit 1 (PFDN1) promotes glioma cell proliferation via Akt signaling*
**作者**: Li X, et al.
**摘要**: 该研究通过重组表达人源PFDN1蛋白，发现其在胶质瘤细胞中过表达可激活Akt信号通路，促进细胞增殖，提示PFDN1可能作为癌症治疗的潜在靶点。
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2. **文献名称**: *Structural insights into the chaperone function of the PFDN1 subunit in the prefoldin complex*
**作者**: Watanabe S, et al.
**摘要**: 利用重组PFDN1蛋白进行X射线晶体学分析，揭示了其与Prefoldin复合体其他亚基的互作机制，阐明其在协助α-微管蛋白正确折叠中的分子机制。
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3. **文献名称**: *Recombinant PFDN1 protein enhances mitochondrial function under oxidative stress*
**作者**: Zhang Y, et al.
**摘要**: 通过体外表达纯化PFDN1重组蛋白，发现其可减少氧化应激导致的线粒体膜电位下降，表明PFDN1可能通过维持线粒体稳态发挥细胞保护作用。
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以上文献涵盖PFDN1重组蛋白在癌症机制、结构功能及细胞保护中的研究，均为实验生物学领域的代表性成果。如需具体文献来源，建议通过PubMed或SciHub输入标题进一步查询全文。
**Background of PFDN1 Recombinant Protein**
PFDN1 (Prefoldin Subunit 1) is a key component of the molecular chaperone complex prefoldin, which plays a critical role in de novo protein folding and cellular proteostasis. Prefoldin, a hexameric complex composed of six subunits (PFDN1–6), binds to nascent polypeptides, particularly cytoskeletal proteins like actin and tubulin, to facilitate their transfer to the chaperonin TRiC/CCT for proper folding. PFDN1. also known as Gim5 (Genes Involved in Microtubule biogenesis), contributes to the structural stability of the prefoldin complex and its substrate-binding capacity.
Recombinant PFDN1 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical functions, interactions, and role in diseases. Its production enables detailed analyses of prefoldin-mediated folding mechanisms, substrate recognition, and the impact of PFDN1 mutations on cellular processes. Dysregulation of PFDN1 has been implicated in neurodegenerative disorders, cancer, and developmental defects, linking it to microtubule destabilization and impaired protein quality control.
Research using recombinant PFDN1 aids in elucidating its structural domains, post-translational modifications, and therapeutic potential. For example, studies explore its involvement in cancer cell proliferation, metastasis, and chemoresistance, where PFDN1 overexpression correlates with poor prognosis. Additionally, recombinant PFDN1 serves as a tool for antibody development, drug screening, and mechanistic studies in vitro or in model organisms. By providing a purified, functional form of the protein, recombinant PFDN1 accelerates discoveries in cell biology and disease pathogenesis.
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