| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Peroxiredoxin 3 |
| Uniprot No | P30048 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 63-256aa |
| 氨基酸序列 | PAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ |
| 预测分子量 | 25.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Mitochondrial peroxiredoxin 3: New insights into its structural and functional characteristics"**
- *Authors: Chae HZ et al.*
- 摘要：研究利用重组PRDX3蛋白解析其晶体结构，揭示其在线粒体抗氧化防御中的催化机制，并探讨其硫氧还蛋白依赖的过氧化物酶活性。
2. **"Recombinant peroxiredoxin 3 protects against oxidative stress-induced apoptosis in neural cells"**
- *Authors: Zhang B et al.*
- 摘要：通过大肠杆菌表达重组PRDX3.验证其清除ROS的能力，并证明其可抑制H₂O₂诱导的神经元凋亡，提示其在神经退行性疾病中的潜在应用。
3. **"Functional characterization of human peroxiredoxin 3 in thyroid cancer cells"**
- *Authors: Kim YJ et al.*
- 摘要：利用重组PRDX3研究其在甲状腺癌细胞中的抗氧化功能，发现其过表达可降低细胞内ROS水平并抑制肿瘤生长，与化疗敏感性相关。
4. **"Redox regulation of mitochondrial peroxiredoxin 3 and its role in apoptosis signaling"**
- *Authors: Cox AG et al.*
- 摘要：通过重组蛋白实验揭示PRDX3通过调控线粒体氧化还原状态影响细胞凋亡通路，尤其在缺血再灌注损伤中的保护作用机制。
(注：以上为模拟文献，实际引用时需核对真实发表信息。)
Peroxiredoxin 3 (PRDX3), a member of the peroxiredoxin family, is a mitochondrial antioxidant enzyme critical for regulating reactive oxygen species (ROS) and maintaining cellular redox homeostasis. As a thioredoxin-dependent peroxidase, it primarily localizes to the mitochondrial matrix, where it scavenges hydrogen peroxide (H₂O₂) and peroxynitrite generated during oxidative phosphorylation. PRDX3 plays a protective role against oxidative stress-induced damage to mitochondrial DNA, proteins, and lipids, thereby influencing apoptosis, metabolism, and cellular signaling pathways. Its dysfunction is linked to neurodegenerative diseases, cancer, aging, and metabolic disorders.
Recombinant PRDX3 protein is engineered using molecular cloning techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. This recombinant form retains enzymatic activity, enabling studies on mitochondrial oxidative stress mechanisms, drug screening, and therapeutic development. Structural analyses reveal its conserved thioredoxin-fold domain and catalytic cysteine residues essential for its peroxidase function. Researchers utilize recombinant PRDX3 to investigate its role in modulating ROS-related diseases, such as Parkinson’s disease, where mitochondrial oxidative damage is a hallmark. Additionally, it serves as a tool to explore PRDX3’s interactions with mitochondrial proteins or its potential as a biomarker. Studies also focus on its redox-sensitive signaling roles in inflammation and cancer progression. The recombinant protein’s stability and activity under varying oxidative conditions make it valuable for in vitro and in vivo models aiming to enhance antioxidant defenses or target mitochondrial dysfunction. Ongoing research explores its therapeutic potential, including gene therapy or small-molecule activators to boost PRDX3 expression in diseases characterized by mitochondrial oxidative stress.
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