首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠补体片断5a(C5a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的C5a含量。C5a是补体系统激活产生的重要成分。其背景在于补体系统是固有免疫关键部分。研究机制方面，C5a可与相应受体结合，参与免疫调节、炎症反应等，异常激活会导致多种炎症相关疾病，因此是炎症性疾病治疗的潜在靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外研究炎症信号通路激活状态、评估药物对补体系统的干预效果，或分析疾病模型中免疫应答动态变化，例如通过检测实验动物血清中C5a浓度变化揭示炎症反应程度，或利用细胞培养上清数据探究特定刺激对补体活化途径的影响，支持科研人员在心血管疾病、神经炎症、免疫调节等基础研究领域开展精准的蛋白定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：C5a
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 102 | ||||
| Range % | 96-108 | |||||
| 1:2000 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4000 | Average % | 96 | ||||
| Range % | 88-99 | |||||
| 1:8000 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-104 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.2412.1402.1912.053 501.8041.7031.7541.616 251.2151.2041.2101.072 12.50.7130.7020.7080.570 6.250.4640.4750.4700.332 3.120.3280.3390.3340.196 1.560.2450.2510.2480.110 00.1370.1380.138 | ||||||
货期：3-5 working days
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