首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠补体蛋白4(C4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C4含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于体外免疫学研究，如炎症动物模型中的血清C4动态监测、组织局部补体激活水平评估，或药物干预下补体通路的功能研究，为免疫学、病理学及药理学领域的科研工作提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C4 ELISA Kit; C4aComplement C4 [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA Kit
缩写：C4
Uniprot No.：P08649
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 96-104 | |||||
| 1:1600 | Average % | 84 | ||||
| Range % | 81-89 | |||||
回收率：
| The recovery of rat C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 88 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.6042.6582.6312.5404002.1852.1982.1922.1012001.6731.6981.6861.5951001.0691.0971.0830.992500.6260.6360.6310.540250.2920.2710.2820.19112.50.1880.1800.1840.09300.0900.0920.091 | ||||||
货期：3-5 working days
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