首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠补体片断3a(C3a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C3a含量。C3a是补体系统的重要成分，由C3蛋白裂解产生，参与免疫和炎症反应。其研究机制涉及C3a与细胞表面受体的相互作用，激活下游信号通路，调节免疫细胞功能。C3a在多种疾病中发挥作用，如糖尿病肾病和多发性骨髓瘤骨病，成为潜在的治疗靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，本试剂盒检测灵敏度覆盖1.56 ng/mL至100 ng/mL宽动态范围，可稳定识别样本中痕量目标蛋白。通过优化的预包被抗体与酶标记检测抗体协同作用，确保检测结果的高特异性和重复性，适用于科研场景中炎症机制研究、免疫相关疾病动物模型评估或药物干预实验中C3a动态变化的监测，为探索补体系统在生理病理中的作用提供可靠工具。实验操作简便，兼容常规实验室设备，配套标准品及检测缓冲液，支持快速完成样本检测与数据分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C3a
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 88 | ||||
| Range % | 80-97 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 92-102 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of rat C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 101 | 95-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.5882.5982.5932.411 501.9481.9031.9261.744 251.4311.4681.4501.268 12.51.0091.0511.0300.848 6.250.6240.6370.6310.449 3.120.4140.4380.4260.244 1.560.2790.2910.2850.103 00.1810.1830.182 | ||||||
货期：3-5 working days
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