首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠补体蛋白3(C3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C3含量。C3即补体成分3，是补体系统的关键分子。它参与固有免疫和适应性免疫，在免疫防御、免疫自稳等方面有重要作用。研究机制上，其激活裂解产生多种活性片段，参与调理吞噬、炎症反应等，异常激活与多种疾病发生发展相关。试剂盒检测范围为0.625 μg/mL-40 μg/mL，该产品可广泛应用于科研领域，包括免疫调控机制研究、炎症性疾病动物模型分析、感染或自身免疫病理过程中补体激活状态的动态监测，以及药理学实验中药物对补体系统影响的评估。其兼容多种样本类型的特点，尤其适用于比较不同组织或体液样本中C3表达水平的变化，为探索补体相关通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C3Complement C3 [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA kit; C3bc ELISA kit; Neutrophil chemotactic factor-2 ELISA kit; ENCF-2); Complement C3 alpha chain; C3a anaphylatoxin ELISA kit; Neutrophil chemotactic factor-1 ELISA kit; ENCF-1); Acylation stimulating protein ELISA kit; ASP ELISA kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA kit
缩写：C3
Uniprot No.：P01026
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 μg/mL-40 μg/mL
灵敏度：0.156 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:400 | Average % | 101 | ||||
| Range % | 97-106 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 84-90 | |||||
回收率：
| The recovery of rat C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 95 | 89-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 400.0850.0830.084 200.1730.1820.178 100.3080.3210.315 50.5760.6080.592 2.50.9830.9610.972 1.251.4621.4651.464 0.6251.8251.8621.844 02.5532.5672.560 | ||||||
货期：3-5 working days
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