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产品描述：人腭/肺/鼻上皮癌关联蛋白4(C20orf186)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C20orf186含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，该产品可应用于基础科研领域，如探究C20orf186在鼻咽癌、肺癌等上皮源性肿瘤中的表达特征，评估其作为潜在生物标志物的相关性，或用于药物干预实验中靶蛋白水平变化的动态监测，为肿瘤发生机制研究及治疗策略开发提供实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BPI fold-containing family B member 4 ELISA Kit; Bpifb4 ELISA Kit; C20orf186 ELISA Kit; dJ726C3.5 ELISA Kit; Gm1006 ELISA Kit; Ligand-binding protein RY2G5 ELISA Kit; Long palate; lung and nasal epithelium carcinoma-associated protein 4 ELISA Kit; LPLC4_HUMAN ELISA Kit; Lplunc4 ELISA Kit
缩写：C20orf186
Uniprot No.：P59827
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C20orf186 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of human C20orf186 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 96-105 | ||||
| EDTA plasma (n=4) | 93 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.9083.1583.0332.8978002.3042.4332.3692.2334001.4781.4751.4771.3412000.6860.7070.6970.5611000.4970.5030.5000.364500.2630.2770.2700.134250.2300.2440.2370.10100.1400.1320.136 | ||||||
货期：3-5 working days
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