Human Biotinidase(BTD) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL002854HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人生物素酰胺酶(BTD)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BTD含量。BTD是一个重要靶点。它在细胞的生理过程中发挥着一定作用。当前研究主要聚焦其在相关信号通路中的作用机制，探寻与疾病发生发展的关联，有望为开发针对BTD的靶向药物、攻克相关疾病提供理论依据。试剂盒检测范围为0.41 ng/ml- 300 ng/ml，灵敏度为0.41 ng/ml。研究者可通过定量分析不同样本中BTD的表达水平，应用于生物素代谢机制研究、遗传性代谢疾病模型构建、营养干预效果评估等基础研究场景，为探索BTD在细胞代谢调控中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Biotinase ELISA Kit; Biotinidase ELISA Kit; Btd ELISA Kit; BTD_HUMAN ELISA Kit; EC 3.5.1.12 ELISA Kit
缩写：
BTD
Uniprot No.：
P43251
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.41 ng/ml - 300 ng/ml
灵敏度：
0.41 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BTD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-106
1:2	Average %	97
	Range %	91-105
1:4	Average %	96
	Range %	89-103
1:8	Average %	101
	Range %	94-108

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
300	2.746	2.634	2.690	2.529
100	2.362	2.267	2.314	2.154
33.33	1.652	1.628	1.640	1.480
11.11	0.933	0.929	0.931	0.770
3.7	0.663	0.641	0.652	0.491
1.23	0.438	0.453	0.445	0.285
0.41	0.259	0.254	0.256	0.096
0	0.156	0.165	0.161