| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCP4L1 |
| Uniprot No | A6NKN8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-68aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSELNTKTSPATNQAAGQEEKGKAGNVKKAEEEEEIDIDLTAPETEK AALAIQGKFRRFQKRKKDPSS |
| 预测分子量 | 10 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设的关于PCP4L1重组蛋白的参考文献示例(实际文献可能较少，建议通过PubMed或Google Scholar查询最新研究)：
1. **文献名称**: *Expression and Functional Characterization of Recombinant PCP4L1 Protein in Neuroblastoma Cells*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了PCP4L1重组蛋白，发现其在神经母细胞瘤细胞中通过调节钙信号通路抑制细胞增殖。
2. **文献名称**: *PCP4L1 Recombinant Protein as a Potential Biomarker in Cerebellar Development*
**作者**: Chen L, et al.
**摘要**: 通过体外实验证实重组PCP4L1蛋白可结合钙调蛋白，提示其在小脑发育中的调控作用，可能作为神经退行性疾病的潜在标记物。
3. **文献名称**: *Structural Insights into PCP4L1 Recombinant Protein Using X-ray Crystallography*
**作者**: Tanaka K, et al.
**摘要**: 首次解析了PCP4L1重组蛋白的晶体结构，揭示了其EF-hand结构域与钙离子结合的关键位点，为功能研究提供结构基础。
**注意**：PCP4L1(Purkinje Cell Protein 4 Like 1)相关研究较少，上述内容为模拟示例。建议结合基因别名(如**PEP19L1**)或功能关键词(如钙结合蛋白)扩展检索，或关注其同源蛋白PCP4的研究获取间接信息。
**Background of PCP4L1 Recombinant Protein**
PCP4L1 (Purkinje Cell Protein 4-Like 1), also known as PEP-19 or PCP4. is a small, highly conserved calcium-binding protein belonging to the PCP4 family. It is primarily expressed in the brain, particularly in cerebellar Purkinje cells, and plays a role in modulating calcium signaling and neuronal excitability. Structurally, PCP4L1 contains an IQ domain that interacts with calmodulin (CaM), enabling it to regulate calcium-dependent processes such as synaptic plasticity, neurotransmitter release, and apoptosis. Its expression is developmentally regulated, suggesting involvement in neural maturation and circuit formation.
Recombinant PCP4L1 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification. This allows researchers to study its biochemical properties, interactions, and functional roles in disease models. Dysregulation of PCP4L1 has been linked to neurological disorders, including Alzheimer’s disease, Huntington’s disease, and schizophrenia, where calcium homeostasis and neuronal survival are disrupted. Additionally, emerging studies suggest its potential role in cancer, as altered expression correlates with tumor progression and metastasis.
The availability of recombinant PCP4L1 facilitates drug discovery, structural studies, and mechanistic insights into calcium-mediated pathways. It also serves as a tool for developing diagnostic biomarkers or therapeutic strategies targeting calcium signaling abnormalities. Ongoing research aims to clarify its precise molecular mechanisms and therapeutic relevance across diverse pathologies.
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