| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCNP |
| Uniprot No | Q8WW12 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-178aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMADGKAGDEKPEKSQRAGAAGGPEEE AEKPVKTKTVSSSNGGESSSRSAEKRSAEEEAADLPTKPTKISKFGFAIG SQTTKKASAISIKLGSSKPKETVPTLAPKTLSVAAAFNEDEDSEPEEMPP EAKMRMKNIGRDTPTSAGPNSFNKGKHGFSDNQKLWERNIKSHLGNVHDQ DN |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCNP(PEST-containing nuclear protein)重组蛋白的模拟参考文献示例(请注意，以下内容为示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Cloning and characterization of human PCNP, a novel nuclear protein containing a PEST sequence"
**作者**: Saito H, et al.
**摘要**: 本研究首次克隆并鉴定了人源PCNP基因，发现其编码含PEST结构域的核蛋白。通过重组蛋白表达，证实PCNP在细胞核内定位，并参与蛋白酶体依赖的蛋白降解通路，提示其可能调控细胞周期进程。
2. **文献名称**: "PCNP promotes tumorigenesis by modulating the ubiquitin-proteasome system in lung cancer"
**作者**: Chen L, et al.
**摘要**: 通过重组PCNP蛋白功能实验，发现其过表达增强肺癌细胞增殖和侵袭能力。机制研究表明PCNP通过泛素-蛋白酶体系统调控关键癌蛋白稳定性，为肺癌治疗提供潜在靶点。
3. **文献名称**: "Recombinant PCNP interacts with DNA repair machinery and enhances genomic stability"
**作者**: Wang Y, et al.
**摘要**: 利用重组PCNP蛋白进行体外结合实验，发现其与BRCA1/2复合物相互作用，促进DNA损伤修复。研究提示PCNP可能通过维持基因组稳定性抑制肿瘤发生。
4. **文献名称**: "Structural insights into PCNP-mediated cell cycle regulation"
**作者**: Zhang R, et al.
**摘要**: 通过X射线晶体学解析重组PCNP蛋白的三维结构，揭示其PEST结构域与CDK2的结合模式，阐明PCNP通过调控CDK2活性影响G1/S期转换的分子机制。
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**建议**：如需真实文献，可通过PubMed、Web of Science等平台搜索关键词“PCNP recombinant protein”“PEST-containing nuclear protein”进行查询。
PCNP (PEST-containing nuclear protein) is a nuclear-localized protein encoded by the *PCNP* gene, first identified for its role in cellular processes such as cell cycle regulation, DNA damage response, and ribosome biogenesis. Structurally, PCNP contains two conserved PEST motifs (rich in proline, glutamic acid, serine, and threonine), which are associated with protein degradation and turnover. It interacts with key regulatory proteins, including MDM2 and p53. suggesting involvement in tumor suppression pathways and apoptosis regulation. Studies highlight its dual role in both promoting and inhibiting cancer progression, depending on cellular context and post-translational modifications.
Recombinant PCNP proteins are engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) to facilitate purification and detection. These proteins serve as critical tools for studying PCNP’s molecular mechanisms, such as its binding partners, enzymatic activities, and subcellular localization. Research using recombinant PCNP has revealed its participation in ribosomal RNA processing via interactions with nucleolar proteins like NPM1. linking it to ribosome assembly and stress responses.
Clinically, PCNP dysregulation is implicated in neuroblastoma, lung cancer, and glioblastoma, where it may act as a biomarker for prognosis or therapeutic targeting. For example, in neuroblastoma, PCNP overexpression correlates with poor survival, while in lung cancer, it modulates chemosensitivity through p53 pathways. Recombinant PCNP enables drug screening and functional assays to explore these pathways, offering potential for developing targeted therapies. Despite progress, its precise molecular functions and regulatory networks remain under investigation, emphasizing the need for further structural and functional studies using recombinant protein-based approaches.
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