首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨形态发生蛋白8B(BMP8B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BMP8B含量。BMP8B属于骨形态发生蛋白家族成员。在背景上，它参与多种生理进程。研究机制方面，其能调控细胞的增殖、分化和凋亡等，在骨代谢、生殖系统等方面发挥作用，深入研究它对理解相关生理病理过程有重要意义。试剂盒检测范围为9.37 pg/mL-600 pg/mL，该产品适用于体外研究BMP8B在骨代谢调控机制、生殖系统功能分析或特定病理模型（如骨发育异常、组织纤维化等）中的动态表达特征，为探索其分子作用机制提供可靠工具。实验操作流程简便，兼容常规实验室设备，支持多样本类型分析，尤其适用于需要高灵敏度检测的细胞或动物模型研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP-8 ELISA Kit; BMP-8B ELISA Kit; BMP8 ELISA Kit; Bmp8b ELISA Kit; BMP8B_HUMAN ELISA Kit; bone morphogenetic protein 8 (osteogenic protein 2) ELISA Kit; Bone morphogenetic protein 8 ELISA Kit; Bone morphogenetic protein 8B ELISA Kit; dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2)) ELISA Kit; OP-2 ELISA Kit; OP2 ELISA Kit; Osteogenic protein 2 ELISA Kit
缩写：BMP8B
Uniprot No.：P34820
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：9.37 pg/mL-600 pg/mL
灵敏度：2.34 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:10 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:20 | Average % | 103 | ||||
| Range % | 99-108 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human BMP8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-100 | ||||
| EDTA plasma (n=4) | 98 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 6002.0562.2052.1312.008 3001.6071.7241.6661.543 1501.1021.0751.0890.966 750.6450.6030.6240.501 37.50.3940.3730.3840.261 18.750.2910.3020.2970.174 9.370.1870.2010.1940.071 00.1200.1250.123 | ||||||
货期：3-5 working days
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