首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨成型蛋白2(BMP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆样本中的BMP2含量。BMP2 即骨形态发生蛋白 2，作为 TGF-β 超家族成员，在胚胎发育、组织修复等生理过程起重要作用。其研究机制主要围绕与受体结合激活信号通路，调控下游基因表达，以促进骨细胞分化、增殖，在骨组织工程和再生医学领域颇具研究价值。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于骨代谢机制研究、组织工程中成骨分化评价、药物或生物材料对BMP - 2通路调控作用分析等科研领域，尤其可用于骨损伤模型、骨质疏松症或骨肿瘤相关研究中BMP - 2的动态监测，为探索其在骨骼疾病治疗及再生医学中的应用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BDA2 ELISA Kit; BMP-2 ELISA Kit; BMP-2A ELISA Kit; Bmp2 ELISA Kit; BMP2_HUMAN ELISA Kit; BMP2A ELISA Kit; Bone morphogenetic protein 2 ELISA Kit; Bone morphogenetic protein 2A ELISA Kit
缩写：BMP2
Uniprot No.：P12643
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-106 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.6732.7862.7302.57220002.3452.3242.3352.17710001.6391.7401.6901.5325001.1801.2001.1901.0322500.8310.8790.8550.6971250.5920.6050.5990.44162.50.3450.3560.3510.19300.1540.1620.158 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BMP2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled BMP2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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