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产品描述：人多梳复合蛋白BMI-1(BMI1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BMI1含量。BMI1是一个重要靶点。它是原癌基因，在调节细胞增殖、分化、衰老及凋亡等方面起关键作用。研究机制上，它参与调控多条重要信号通路，如抑制p16等肿瘤抑制基因表达。异常表达与多种肿瘤发生、发展密切相关，成为抗癌药物研发焦点。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于肿瘤生物学、干细胞分化等基础研究领域，可精准检测不同实验条件下BMI1的蛋白表达水平变化，为探索肿瘤发生机制、评估靶向药物干预效果提供可靠工具，广泛适用于细胞模型、动物模型及临床前样本的蛋白定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B lymphoma Mo MLV insertion region (mouse) ELISA Kit; B lymphoma Mo MLV insertion region 1 homolog ELISA Kit; Bmi 1 ELISA Kit; BMI1 ELISA Kit; BMI1 polycomb ring finger oncogene ELISA Kit; BMI1_HUMAN ELISA Kit; Flvi 2/bmi 1 ELISA Kit; FLVI2/BMI1 ELISA Kit; MGC12685 ELISA Kit; Murine leukemia viral (bmi 1) oncogene homolog ELISA Kit; Oncogene BMI 1 ELISA Kit; PCGF 4 ELISA Kit; PCGF4 ELISA Kit; Polycomb complex protein BMI 1 ELISA Kit; Polycomb complex protein BMI-1 ELISA Kit; Polycomb group protein Bmi1 ELISA Kit; Polycomb group ring finger 4 ELISA Kit; Polycomb group RING finger protein 4 ELISA Kit; RING finger protein 51 ELISA Kit; RNF 51 ELISA Kit; RNF51 ELISA Kit
缩写：BMI1
Uniprot No.：P35226
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 99-107 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
回收率：
| The recovery of human BMI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 80-89 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16001.7681.8961.8321.7148001.1691.2031.1861.0684000.7390.7040.7220.6042000.4720.4570.4650.3471000.3200.3400.3300.212500.2670.2710.2690.151250.1950.1820.1890.07100.1210.1150.118 | |||||||
货期：3-5 working days
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