| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCBP1 |
| Uniprot No | Q15365 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-163aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMDAGVTESGLNVTLTIRLLMHGKEVGS IIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIID KLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIREST GAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQIC |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCBP1重组蛋白的3篇模拟参考文献示例(基于公开研究领域知识总结，非真实文献)：
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1. **文献名称**: "Poly(rC)-Binding Protein 1 (PCBP1) Regulates mRNA Stability Through Interaction with Iron-Responsive Elements"
**作者**: Xu, M.; et al.
**摘要**: 本研究通过重组表达人源PCBP1蛋白，揭示了其通过结合RNA中的铁响应元件(IREs)调控转铁蛋白受体(TfR)mRNA稳定性的分子机制，表明PCBP1在细胞铁代谢中起关键作用。
2. **文献名称**: "Structural Insights into PCBP1-DNA/RNA Binding Domains by X-ray Crystallography"
**作者**: Wang, L.; Zhang, Y.
**摘要**: 通过重组PCBP1蛋白的KH结构域结晶分析，解析了其与DNA/RNA结合的分子结构，揭示了PCBP1多价结合核酸的机制，为设计靶向PCBP1的分子工具奠定基础。
3. **文献名称**: "PCBP1 Recombinant Protein Suppresses Tumor Growth by Modulating Alternative Splicing in Hepatocellular Carcinoma"
**作者**: Choi, Y.; et al.
**摘要**: 利用重组PCBP1蛋白处理肝癌细胞，发现其通过调控致癌基因(如EGFR)的可变剪接抑制肿瘤增殖，提示PCBP1可能作为癌症治疗的潜在靶点。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词 **"PCBP1 recombinant protein"** 或 **"PCBP1 expression and function"**，并筛选近年高影响力论文。
**Background of PCBP1 Recombinant Protein**
Poly(rC)-binding protein 1 (PCBP1), also known as hnRNP E1 or αCP1. is a multifunctional RNA-binding protein belonging to the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It contains three K-homology (KH) domains that enable binding to single-stranded nucleic acids, particularly poly(C)-rich sequences. PCBP1 plays critical roles in post-transcriptional gene regulation, including mRNA stability, translation initiation, and RNA splicing. It is also involved in iron metabolism by interacting with iron regulatory proteins (IRPs) to regulate ferritin translation.
The recombinant PCBP1 protein is engineered for research applications, typically produced via bacterial expression systems (e.g., *E. coli*) with tags such as His-tag for purification. Its recombinant form retains structural and functional integrity, allowing in vitro studies to dissect molecular mechanisms. Researchers use PCBP1 recombinant protein to investigate its interactions with RNA/DNA, its role in cellular stress responses (e.g., oxidative stress), and its involvement in viral replication processes, where it may bind viral RNA to modulate infection.
PCBP1's dysregulation has been linked to cancers, neurodegenerative diseases, and iron-related disorders, making its recombinant variant a valuable tool for therapeutic target exploration. Studies also highlight its participation in DNA repair, apoptosis, and immune signaling, underscoring its broad biological significance. By leveraging recombinant PCBP1. scientists aim to unravel its pleiotropic functions and potential applications in biotechnology and medicine.
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