首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人博来霉素水解酶(BLMH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BLMH含量。BLMH（博莱霉素水解酶）是一种在进化中高度保守的胞质半胱氨酸肽酶，主要功能是代谢失活抗肿瘤药物博莱霉素（BLM），保护细胞免受其毒性作用。其研究机制涉及对BLM的羧酰胺键进行水解，维持细胞平衡和防御有害物质。试剂盒检测范围为28 pg/mL-1800 pg/mL，适用于体外培养细胞模型、动物组织样本或生物体液中的BLMH蛋白定量分析，为探索其在细胞应激调控、药物代谢机制研究、疾病相关分子标志物筛选等科研方向提供精准检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BH ELISA Kit; Bleomycin hydrolase ELISA Kit; BLM hydrolase ELISA Kit; BLMH ELISA Kit; BLMH_HUMAN ELISA Kit; BMH ELISA Kit; EC 3.4.22.40 ELISA Kit
缩写：BLMH
Uniprot No.：Q13867
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BLMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 110 | ||||
| Range % | 107-113 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 83-89 | |||||
回收率：
| The recovery of human BLMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-99 | ||||
| EDTA plasma (n=4) | 105 | 101-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18002.3642.3292.3472.2259001.5261.5681.5471.4254500.9610.9750.9680.8462250.5920.5780.5850.463112.50.3830.3720.3780.256560.2980.2710.2850.163280.1970.1890.1930.07100.1210.1230.122 | ||||||
货期：3-5 working days
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