Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E05129r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠骨钙素/骨谷氨酸蛋白(OT/BGP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆.样本中的BGLAP含量。BGLAP是重要靶点。它与骨代谢密切相关，在成骨细胞中表达，是反映骨形成的特异指标。研究机制方面，聚焦其在骨发育、骨重建等进程中的作用，探索调节它的信号通路，有望为骨相关疾病如骨质疏松症等治疗提供新思路。试剂盒检测范围为125 pg/mL- 8000 pg/mL，该产品适用于骨代谢基础研究、骨质疏松动物模型评估、骨修复药物筛选等科研场景，为探究骨生物学机制及药物干预效果提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Bglap ELISA Kit; Bglap2 ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
缩写：
BGLAP
Uniprot No.：
P04640
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma,tissue homogenates.
检测范围：
125 pg/mL- 8000 pg/mL
灵敏度：
31.25 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-107
1:2	Average %	97
	Range %	94-101
1:4	Average %	97
	Range %	92-102
1:8	Average %	96
	Range %	89-101

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
8000	1.947	1.834	1.891	1.713
4000	1.218	1.167	1.193	1.015
2000	0.682	0.658	0.670	0.493
1000	0.453	0.429	0.441	0.264
500	0.292	0.301	0.296	0.119
250	0.233	0.243	0.238	0.061
125	0.202	0.194	0.198	0.020
0	0.180	0.175	0.177