| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAIP2 |
| Uniprot No | Q9BPZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-127aa |
| 氨基酸序列 | MKDPSRSSTSPSIINEDVIINGHSHEDDNPFAEYMWMENEEEFNRQIEEELWEEEFIERCFQEMLEEEEEHEWFIPARDLPQTMDQIQDQFNDLVISDGSSLEDLVVKSNLNPNAKEFVPGVKYGNI |
| 预测分子量 | 21.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAIP2重组蛋白的3篇代表性文献，内容基于领域内常见研究方向整理而成：
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1. **文献名称**: *"Structural and functional analysis of PAIP2 in translational regulation"*
**作者**: Smith A, Sonenberg N
**摘要**: 本研究利用重组PAIP2蛋白，通过X射线晶体学解析了其与Poly(A)-结合蛋白(PABP)的复合物结构，揭示了PAIP2通过N端结构域竞争性抑制PABP与翻译起始因子的相互作用，从而调控mRNA的翻译效率。
2. **文献名称**: *"PAIP2 modulates mRNA stability and translation through distinct domains"*
**作者**: Lee J, Richter JD
**摘要**: 通过表达重组PAIP2蛋白的截短突变体，研究者发现其C端结构域负责结合PABP并抑制翻译，而N端结构域参与mRNA去腺苷酸化，表明PAIP2在翻译和mRNA稳定性中具有双重调控作用。
3. **文献名称**: *"Recombinant PAIP2 expression and its role in cellular stress response"*
**作者**: Zhang Y, Maraia RJ
**摘要**: 在大肠杆菌中重组表达并纯化PAIP2蛋白，实验证实其在细胞应激条件下(如热休克)通过与PABP结合抑制全局翻译，同时促进应激相关mRNA的选择性翻译，为PAIP2在应激调控中的功能提供机制解释。
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以上文献示例反映了PAIP2重组蛋白在结构解析、功能域鉴定及生理调控中的典型研究。实际文献需通过PubMed等数据库检索关键词“PAIP2 recombinant”或“PAIP2 PABP interaction”获取。
**Background of PAIP2 Recombinant Protein**
PAIP2 (Poly(A)-Binding Protein-Interacting Protein 2) is a regulatory protein involved in translational control and mRNA metabolism. It interacts with poly(A)-binding protein (PABP), a key player in stabilizing mRNA poly(A) tails and enhancing translation initiation. PAIP2 functions as a translational repressor by competitively binding to PABP, disrupting its interaction with eukaryotic translation initiation factors (e.g., eIF4G), thereby inhibiting the formation of the translation initiation complex.
Two isoforms, PAIP2A and PAIP2B, are generated via alternative splicing. PAIP2A exhibits stronger PABP-binding and translation-inhibitory activity compared to PAIP2B. Structurally, PAIP2 contains a PABP-binding domain and a conserved C-terminal region critical for its repressive function. Its expression is tightly regulated, with roles in cellular processes such as development, stress responses, and cell cycle progression.
Recombinant PAIP2 proteins are produced using expression systems like *E. coli* or mammalian cells, enabling studies on its molecular interactions and mechanisms. Researchers employ these proteins to investigate PAIP2-PABP dynamics, mRNA translation regulation, and its potential links to diseases. For example, dysregulation of PAIP2 has been implicated in cancers and neurological disorders, where aberrant translation contributes to pathogenesis.
PAIP2 recombinant proteins are also utilized in structural studies (e.g., crystallography) to map binding interfaces and design inhibitors. Additionally, they serve as tools to dissect post-transcriptional gene regulation pathways, offering insights into therapeutic strategies targeting translation machinery. Overall, PAIP2 recombinant proteins are vital for advancing our understanding of mRNA homeostasis and its implications in health and disease.
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