首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠β2微球蛋白(BMG/β2-MG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的B2M含量。B2M即β2-微球蛋白，是MHCⅠ类分子的轻链部分，广泛存在于有核细胞表面。其在免疫识别、抗原呈递中发挥重要作用。研究机制围绕免疫调节展开，如它影响T细胞对靶细胞的识别。此外，其表达异常与多种疾病如肿瘤、自身免疫病相关。试剂盒检测范围为0.156 μg/mL-10 μg/mL，适用于探索肾脏生理病理机制、评估药物肾毒性、肿瘤免疫微环境分析等基础研究领域，尤其为尿液样本的肾小管功能研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B2MBeta-2-microglobulin ELISA kit
缩写：B2M
Uniprot No.：P01885
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.156 μg/mL-10 μg/mL
灵敏度：0.039 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-104 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected102.6452.6782.6622.51551.8551.8961.8761.7292.51.2561.2631.2601.1131.250.8340.8690.8520.7050.6250.4870.4890.4880.3410.3120.3250.3530.3390.1920.1560.2610.2840.2730.12600.1450.1490.147 | ||||||
货期：3-5 working days
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