首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Aβ1-42含量。Aβ1-42是重要的阿尔茨海默病相关靶点。其在脑内异常聚集形成淀粉样斑块，被认为是该病发病的关键病理特征。研究聚焦于其生成、清除机制、神经毒性作用等，期望通过干预Aβ1-42代谢过程开发有效治疗手段。试剂盒检测范围为0.9 pg/mL-60 pg/mL，适用于神经科学领域的基础研究，如阿尔茨海默病动物模型中Aβ1 - 42的动态变化观察、药物干预效果评价或病理机制探索，其宽泛的样本兼容性可满足脑组织、血液等多种生物样本的检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
缩写：Aβ1-42
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 pg/mL-60 pg/mL
灵敏度：0.225 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected602.2812.2742.2782.136301.5991.5761.5881.446150.9670.9240.9460.8047.50.5380.5280.5330.3913.750.3950.3710.3830.2411.80.3210.3120.3170.1750.90.2350.2500.2430.10100.1410.1430.142 | ||||||
货期：3-5 working days
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