| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAEP |
| Uniprot No | P09466 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180aa |
| 氨基酸序列 | MLCLLLTLGVALVCGVPAMDIPQTKQDLELPKLAGTWHSMAMATNNISLM ATLKAPLRVHITSLLPTPEDNLEIVLHRWENNSCVEKKVLGEKTENPKKF KINYTVANEATLLDTDYDNFLFLCLQDTTTPIQSMMCQYLARVLVEDDEI MQGFIRAFRPLPRHLWYLLDLKQMEEPCRF |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAEP(孕酮相关子宫内膜蛋白，也称Glycodelin)重组蛋白研究的参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant Human PAEP in a Mammalian Cell System"*
**作者**：Zhang L, et al.
**摘要**：本研究成功在HEK293细胞中表达了重组人PAEP蛋白，并通过亲和层析纯化。功能实验表明，重组PAEP可显著抑制子宫内膜细胞中T淋巴细胞的增殖，提示其在母胎免疫耐受中的潜在作用。
2. **文献名称**：*"Glycodelin-A as a Immune Modulator: Insights from Recombinant Protein Studies"*
**作者**：Kovář J, et al.
**摘要**：作者利用昆虫细胞表达系统制备重组Glycodelin-A(PAEP)，发现其能通过结合CD4+ T细胞表面的受体抑制IL-2分泌，为开发免疫相关疾病的治疗策略提供了依据。
3. **文献名称**：*"Structural Analysis of Recombinant PAEP Reveals Key Domains for Progesterone Binding"*
**作者**：Santos C, et al.
**摘要**：通过X射线晶体学解析重组PAEP的三维结构，发现其N端结构域是孕酮结合的关键区域，为理解PAEP在生殖生理中的作用机制提供了结构基础。
4. **文献名称**：*"Development of a PAEP-Specific ELISA Using Recombinant Protein for Endometrial Receptivity Assessment"*
**作者**：Wang Y, et al.
**摘要**：研究利用大肠杆菌表达的重组PAEP制备多克隆抗体，并建立高灵敏度ELISA检测方法，用于评估女性子宫内膜容受性，显示其在辅助生殖技术中的临床应用潜力。
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**提示**：以上为模拟参考文献，实际文献检索建议通过PubMed、Web of Science等平台以关键词“PAEP recombinant”“Glycodelin expression”查询。真实研究多涉及PAEP在生殖、免疫调节或癌症中的功能探索。
**Background of PAEP Recombinant Protein**
Progestagen-Associated Endometrial Protein (PAEP), also known as glycodelin or progesterone-associated endometrial protein, is a glycoprotein primarily expressed in reproductive tissues, including the endometrium, ovary, and placenta. It plays critical roles in regulating female reproductive processes, such as embryo implantation, immunomodulation during early pregnancy, and endometrial differentiation. PAEP is encoded by the *PAEP* gene and exists in multiple isoforms, with glycodelin-A (secreted in the endometrium) and glycodelin-F (found in follicular fluid) being the most studied. These isoforms exhibit structural variations in glycosylation patterns, influencing their biological functions, including immunosuppression, cell adhesion, and apoptosis regulation.
The recombinant PAEP protein is produced using genetic engineering techniques, often expressed in systems like *E. coli* or mammalian cell cultures to ensure proper folding and post-translational modifications. Recombinant technology enables large-scale production of standardized, high-purity PAEP for research and therapeutic applications. Studies utilizing recombinant PAEP have elucidated its dual roles in reproduction and pathology. For instance, it suppresses natural killer (NK) cell activity to protect the semi-allogeneic fetus during pregnancy but is also implicated in tumor progression (e.g., breast and ovarian cancers) by promoting immune evasion.
PAEP's expression is hormonally regulated, peaking during the secretory phase of the menstrual cycle and early gestation. Dysregulated PAEP levels are linked to infertility, recurrent miscarriages, and cancer, making it a potential biomarker or therapeutic target. Recombinant PAEP serves as a vital tool for investigating its molecular mechanisms, developing diagnostic assays, and exploring therapeutic strategies in reproductive health and oncology. Ongoing research aims to harness its immunomodulatory properties for applications in autoimmune diseases and cancer immunotherapy.
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