| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARF3 |
| Uniprot No | P61204 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-181aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGNIFGNLLKSLIGKKEMRILMVGLDAAGK TTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRH YFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDL PNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLANQLKNK K |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARF3重组蛋白的3篇参考文献示例(注：文献为虚构示例，仅供参考格式和内容框架)：
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1. **文献名称**：*"Recombinant ARF3 expression in E. coli and its role in auxin response modulation"*
**作者**：Zhang L, Wang Q, et al.
**摘要**：本研究成功克隆拟南芥ARF3基因并在大肠杆菌中表达重组蛋白，通过Ni-NTA层析纯化获得高纯度ARF3.体外实验表明，重组ARF3可特异性结合生长素响应元件(AuxRE)，并激活下游报告基因表达，揭示了其在生长素信号通路中的直接作用。
2. **文献名称**：*"Crystal structure of the auxin response factor ARF3 DNA-binding domain"*
**作者**：Thompson R, Silva AT, et al.
**摘要**：通过昆虫细胞系统表达并纯化ARF3的DNA结合域(DBD)重组蛋白，利用X射线晶体学解析其三维结构。结构分析显示，ARF3 DBD通过保守的B3结构域与DNA相互作用，为理解ARF家族蛋白的靶向识别机制提供结构基础。
3. **文献名称**：*"ARF3 recombinant protein facilitates in vitro analysis of protein-protein interactions in vesicle trafficking"*
**作者**：Kumar S, Ito E, et al.
**摘要**：研究利用哺乳动物HEK293细胞系统表达带标签的ARF3重组蛋白，结合Pull-down实验证实其与COPⅠ囊泡组分Sec21的直接互作，表明ARF3在调控胞内运输中的潜在功能。
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**说明**：以上文献为模拟示例，实际研究中需通过学术数据库(如PubMed、Web of Science)检索真实文献。建议使用关键词“ARF3 recombinant protein expression”“ARF3 structure/function”等进一步查询。
**Background of ARF3 Recombinant Protein**
ADP-ribosylation factor 3 (ARF3) is a member of the ARF family of small GTP-binding proteins, which play critical roles in regulating intracellular vesicular trafficking, cytoskeletal organization, and signal transduction. As part of the Ras superfamily, ARF3 cycles between an inactive GDP-bound state and an active GTP-bound state, acting as a molecular switch to control membrane recruitment of effector proteins, such as coat proteins and lipid-modifying enzymes, during vesicle formation and cargo transport.
ARF3 is ubiquitously expressed and localizes primarily to the Golgi apparatus and endosomal compartments, where it facilitates retrograde transport between the Golgi and endoplasmic reticulum. Dysregulation of ARF3 has been implicated in pathological conditions, including cancer metastasis and neurological disorders, due to its role in maintaining cellular homeostasis and secretory pathways.
Recombinant ARF3 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce high-purity, functional protein for *in vitro* studies. This tool enables researchers to investigate ARF3’s GTPase activity, interactions with guanine nucleotide exchange factors (GEFs) or GTPase-activating proteins (GAPs), and its regulatory mechanisms in membrane dynamics. Additionally, recombinant ARF3 is utilized in drug discovery to screen for inhibitors or activators targeting ARF-mediated pathways, offering potential therapeutic avenues for diseases linked to trafficking defects. Its study continues to shed light on fundamental cell biology and disease mechanisms.
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