关键信息
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基因名
Gastric lipase
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简介
Gastric lipase is a key enzyme in the digestive process and plays a key role in the hydrolysis of triacylglycerols into free fatty acids, diacylglycerols, monoacylglycerols, and glycerol. This enzyme activity contributes significantly to the breakdown of dietary fat in the gastric environment. Gastric lipase Protein, Human (HEK293, His) is the recombinant human-derived Gastric lipase protein, expressed by HEK293 , with C-6*His labeled tag.
- 应用
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别名
rHuGastric triacylglycerol lipase/LIPF, His ; Gastric Triacylglycerol Lipase; GL; Gastric Lipase; LIPF
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
AAI12273.1
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表达区间
L20-K398
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氨基酸序列
LFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKLRFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQDVGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISEDKK
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蛋白长度
Partial
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分子量
50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Gastric lipase is a crucial enzyme primarily produced in the stomach, playing a vital role in the digestion of dietary fats. Its primary function is to hydrolyze triglycerides into free fatty acids and diglycerides, which facilitates fat absorption in the intestines. Recent studies have shown that gastric lipase not only contributes to lipid digestion but also has potential implications in metabolic disorders and obesity, making it a subject of significant interest in the field of nutrition and health. However, the study of gastric lipase has been limited due to challenges in obtaining pure and active enzyme samples. Recombinant protein technology has emerged as a valuable method to produce gastric lipase in larger quantities and with enhanced purity. This approach allows for detailed biochemical characterization and functional analysis, offering insights into its enzymatic mechanisms and potential therapeutic applications. Furthermore, recombinant gastric lipase can be utilized in various industrial applications, including food processing and pharmaceutical formulations. As research progresses, understanding gastric lipase's structure-function relationship will pave the way for innovative strategies to target lipid metabolism disorders, enhancing human health and well-being.
