| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human INSIG2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与INSIG2抗体相关的文献示例(内容为虚构模拟，仅作格式参考)：
1. **文献名称**: "INSIG2 modulates hepatic lipid metabolism through SREBP-1 interaction"
**作者**: Yabe D, et al.
**摘要**: 通过Western blot和免疫共沉淀技术，利用INSIG2抗体揭示其在肝脏中通过结合SREBP-1调控脂质合成的分子机制，为代谢综合征研究提供依据。
2. **文献名称**: "INSIG2 expression in human hepatocellular carcinoma"
**作者**: Yang L, et al.
**摘要**: 使用INSIG2抗体的免疫组化分析显示，INSIG2在肝癌组织中的表达显著下调，提示其可能作为抑癌基因参与肝癌进展及脂代谢异常。
3. **文献名称**: "Role of INSIG2 in obesity-associated insulin resistance"
**作者**: Lee SJ, Smith JD.
**摘要**: 通过INSIG2抗体的蛋白检测发现，肥胖小鼠脂肪组织中INSIG2表达降低，干扰胆固醇反馈调节通路，可能与胰岛素抵抗的发生相关。
4. **文献名称**: "INSIG2 antibody-based detection in neurodegenerative models"
**作者**: Johnson R, et al.
**摘要**: 利用特异性INSIG2抗体，研究阿尔茨海默病模型小鼠脑组织中的脂代谢异常，发现INSIG2与Aβ沉积存在潜在关联。
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注：以上文献信息为示例，实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar以“INSIG2 antibody”为关键词检索近期研究。
**Background of INSIG2 Antibody**
The insulin-induced gene 2 (INSIG2) encodes a protein that plays a critical role in regulating cholesterol and lipid metabolism. INSIG2. along with INSIG1. is an endoplasmic reticulum (ER)-resident membrane protein involved in feedback control of sterol regulatory element-binding protein (SREBP) pathways. By interacting with SREBP cleavage-activating protein (SCAP), INSIG2 retains the SCAP-SREBP complex in the ER under high sterol conditions, blocking proteolytic activation of SREBPs and suppressing genes responsible for cholesterol and fatty acid synthesis.
INSIG2 antibodies are essential tools for studying its expression, localization, and function in metabolic regulation. These antibodies enable detection of INSIG2 in various experimental setups, including Western blotting, immunohistochemistry, and immunofluorescence. Research has linked INSIG2 polymorphisms to obesity, type 2 diabetes, and cancer, highlighting its clinical relevance. Studies using INSIG2 antibodies have also explored its role in lipid homeostasis, drug responses (e.g., statins), and crosstalk with insulin signaling pathways.
Due to its tissue-specific _expression (e.g., liver, adipose tissue) and dynamic regulation by nutrients/hormones, INSIG2 antibodies aid in understanding metabolic diseases and potential therapeutic targets. Their specificity and validation in different species/models remain crucial for reliable data interpretation.
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