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Human epidermal growth factor receptor,EGFR ELISA Kit

  • 中文名: 人表皮生长因子受体(EGFR)ELISA Kit
货号: IPD-E12124h
Price: ¥2500
数量:
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产品详情

  • 产品描述:人表皮生长因子受体(EGFR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的EGFR含量。EGFR即表皮生长因子受体,是一种跨膜蛋白,在细胞生长、增殖和分化中起重要作用。其异常激活与多种癌症发生发展密切相关。研究聚焦于其信号通路机制,通过阻断信号传导,研发靶向药物抑制肿瘤生长,为癌症治疗提供新思路。试剂盒检测范围为0.312 ng/mL-20 ng/mL,灵敏度为0.019 ng/mL。该产品适用于基础医学研究中对EGFR表达水平的动态监测,如肿瘤微环境分析、药物干预效果评估或分子信号通路研究,也可用于生物标志物筛选及疾病机制探索本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:Avian erythroblastic leukemia viral (v erb b) oncogene homolog ELISA Kit; Cell growth inhibiting protein 40 ELISA Kit; Cell proliferation inducing protein 61 ELISA Kit; EGF R ELISA Kit; EGFR ELISA Kit; EGFR_HUMAN ELISA Kit; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) ELISA Kit; Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) ELISA Kit; Epidermal growth factor receptor ELISA Kit; erb-b2 receptor tyrosine kinase 1 ELISA Kit; ERBB ELISA Kit; ERBB1 ELISA Kit; Errp ELISA Kit; HER1 ELISA Kit; mENA ELISA Kit; NISBD2 ELISA Kit; Oncogen ERBB ELISA Kit; PIG61 ELISA Kit; Proto-oncogene c-ErbB-1 ELISA Kit; Receptor tyrosine protein kinase ErbB 1 ELISA Kit; Receptor tyrosine-protein kinase ErbB-1 ELISA Kit; SA7 ELISA Kit; Species antigen 7 ELISA Kit; Urogastrone ELISA Kit; v-erb-b Avian erythroblastic leukemia viral oncogen homolog ELISA Kit; wa2 ELISA Kit; Wa5 ELISA Kit

  • 缩写:EGFR

  • Uniprot No.:P00533

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:0.312 ng/mL-20 ng/mL

  • 灵敏度:0.019 ng/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Signal Transduction

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human EGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)

    1:5Average %101

    Range %95-104

    1:10Average %99

    Range %91-103

    1:20Average %94

    Range %87-98

    1:40Average %108

    Range %97-112

  • 回收率:

    The recovery of human EGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)10396-107

    EDTA plasma (n=4)9384-97

















  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/mlOD1OD2AverageCorrected  202.5222.4632.4932.375  102.0181.9771.9981.880  51.4081.4631.4361.318  2.51.0511.0251.0380.920  1.250.6660.6470.6570.539  0.6250.4270.4030.4150.297  0.3120.2640.2610.2630.145  00.1190.1170.118   







  • 货期:3-5 working days

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