首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人维生素B12(VB12)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的VB12含量。VB12即维生素B12，是人体必需的水溶性维生素。它参与神经系统发育、造血等重要生理过程。研究机制上，它作为辅酶参与甲基转移反应和L-甲基丙二酰辅酶A异构化反应，缺乏VB12会导致巨幼细胞贫血、神经系统损害等疾病。试剂盒检测范围为1.56 pg/mL-100 pg/mL，灵敏度为1.498 pg/mL。适用于细胞培养、动物模型或生物样本中的VB12动态监测，支持营养干预研究、代谢性疾病机制探索及药物开发中的生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：VB12
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：1.498 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human VB12 antibody.
Standard (2 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-conjugated VB12(100 x concentrate) (1 x 600 μl)
HRP-avidin (100 x concentrate) (1 x 120 μl)
Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated VB12
HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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