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产品描述：犬转移生长因子β1(TGF-β1)酶联免疫试剂盒检测血清、血浆样本中的TGFB1含量。TGFB1 即转化生长因子 -β1，在细胞生长、分化、免疫调节等多种生理过程中扮演重要角色。其异常表达与多种疾病相关，如肿瘤、纤维化等。研究机制聚焦于其信号通路，探索通过干预该通路开发治疗疾病的方法。试剂盒检测范围为0.781 ng/ml- 50 ng/ml，灵敏度为0.747 ng/ml。适用于动物实验研究中炎症反应评估、纤维化疾病模型构建、免疫调节机制探索等科研领域，为犬类疾病机理研究及药物干预效果评价提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写：TGFB1
Uniprot No.：P54831
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma
检测范围：0.781 ng/ml - 50 ng/ml
灵敏度：0.747 ng/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 84 | |
| Range % | 81-89 | ||
| 1:2 | Average % | 99 | |
| Range % | 94-103 | ||
| 1:4 | Average % | 87 | |
| Range % | 84-91 | ||
| 1:8 | Average % | 100 | |
| Range % | 94-106 |
回收率：
| The recovery of dog TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 97 | 94-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.4082.4412.4252.285 252.2822.1002.1912.051 12.51.8311.8981.8651.725 6.251.3721.3981.3851.245 3.1250.7180.7320.7250.585 1.5630.4020.4120.4070.267 0.7810.2510.2500.2510.111 00.1410.1390.140 | ||||||
货期：3-5 working days
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