首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠睾酮(T)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的T含量。大鼠睾酮（T）是男性激素，对生殖功能和男性特征发育至关重要。其合成主要在睾丸间质细胞（Leydig细胞）中进行，受促性腺激素（LH）调控。睾酮水平异常与多种生殖和生理问题相关，如少弱精子症、性功能障碍等。研究睾酮的作用机制有助于开发治疗相关疾病的药物和方法。试剂盒检测范围为0.13 ng/mL-25.6 ng/mL，灵敏度为0.06 ng/mL。动物实验研究中的激素水平监测，如生殖生物学机制探索、药物或环境因素对内分泌系统的影响评估、以及疾病模型（如代谢综合征或性腺功能障碍）的睾酮动态变化分析等领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：T
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.13 ng/mL-25.6 ng/mL
灵敏度：0.06 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-88 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-93 | |||||
回收率：
| The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 25.60.2380.2360.237 6.40.4350.4310.433 20.6110.6150.613 0.50.9840.9820.983 0.131.4031.3981.401 02.0012.0032.002 | ||||||
本试剂盒所含材料：
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A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit Testosterone antibody.
Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
Anti-Testosterone Antibody 1 x 6 ml -- Act as the detection antibody.
HRP-conjugated Testosterone 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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