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Human Pentraxin 3 (PTX3)ELISA Kit

  • 中文名： 人五聚素3 (PTX3)ELISA Kit
货号: IPD-E12926h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人五聚素3 (PTX3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTX3含量。PTX3（正五聚蛋白3）是一种长链PTX，位于染色体3q25，由TNF-α和IL-1等炎症介质诱导产生。它作为可溶性模式识别分子，参与机体的致病过程，与炎症反应、免疫应答、细胞凋亡等多种生物学功能密切相关。PTX3在自身免疫性疾病中发挥重要作用，如类风湿关节炎、大动脉炎、狼疮性肾炎等。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。该产品适用于基础科研中探索炎症相关疾病机制、评估心血管病理模型中的生物标志物动态变化、分析感染或创伤后的免疫应答调控，以及药物开发过程中对PTX3靶向干预效果的体外评价，为研究者提供稳定可靠的工具支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AI607804 ELISA Kit; Pentaxin 3 ELISA Kit; Pentaxin-related protein PTX3 ELISA Kit; Pentraxin 3 long ELISA Kit; Pentraxin-related gene ELISA Kit; Pentraxin-related gene rapidly induced by IL-1 beta ELISA Kit; Pentraxin-related protein PTX3 ELISA Kit; PTX3 ELISA Kit; PTX3_HUMAN ELISA Kit; TNF alpha-induced protein 5 ELISA Kit; TNFAIP5 ELISA Kit; TSG-14 ELISA Kit; TSG14 ELISA Kit; Tumor necrosis factor alpha-induced protein 5 ELISA Kit; Tumor necrosis factor-inducible gene 14 protein ELISA Kit; Tumor necrosis factor-inducible protein TSG-14 ELISA Kit
  • 缩写：
    PTX3
  • Uniprot No.：
    P26022
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/ml - 5000 pg/ml
  • 灵敏度：
    19.5 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)597.932625.932609.582621.333605.896618.612SD0.0370.0450.0420.0550.0500.057CV(%)3.6104.2614.0465.2334.8365.439
  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human PTX3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Plasma (n=4)


    1:1

    Average %

    88


    Range %

    80-96


    1:2

    Average %

    95


    Range %

    91-100


    1:4

    Average %

    92


    Range %

    87-98


    1:8

    Average %

    93


    Range %

    85-98


  • 回收率：

    The recovery of human PTX3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    EDTA plasma (n=4)

    90

    86-95


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.525

    2.577

    2.551

    2.344


    2500

    2.167

    2.127

    2.147

    1.940


    1250

    1.614

    1.593

    1.604

    1.397


    625

    1.024

    0.997

    1.011

    0.804


    312

    0.720

    0.713

    0.717

    0.510


    156

    0.452

    0.473

    0.463

    0.256


    78

    0.324

    0.349

    0.337

    0.130


    0

    0.201

    0.213

    0.207



  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PTX3 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled PTX3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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