Human Plasminogen,Plg ELISA Kit

中文名： 人纤溶酶原(Plg)ELISA Kit

货号: IPD-E09177h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人纤溶酶原(Plg)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLG含量。PLG即纤溶酶原，是纤溶系统的关键成分。它可被激活转变为纤溶酶，参与降解纤维蛋白凝块等过程。在研究机制上，围绕其激活途径、与其他因子相互作用开展，对血栓溶解、肿瘤侵袭转移等方面研究有重要意义。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，灵敏度为15.6 ng/mL。适用于心血管疾病机制研究、纤维化病理模型评估、肿瘤微环境分析等科研领域，为探索 Plg 在止血平衡、炎症反应及组织再生中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Plasmin ELISA Kit; Plasmin heavy chain A ELISA Kit; Plasmin light chain B ELISA Kit; Plasminogen ELISA Kit; PLG ELISA Kit; PLMN_HUMAN ELISA Kit
缩写：
PLG
Uniprot No.：
P00747
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
62.5 ng/mL-4000 ng/mL
灵敏度：
15.6 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	526.706	537.081	507.897	509.272	485.791	498.025
SD	0.048	0.049	0.053	0.048	0.054	0.057
CV(%)	5.955	6.164	6.378	5.790	6.236	6.738

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Plg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:100

Average %

101

Range %

96-107

1:200

Average %

105

Range %

99-109

1:400

Average %

Range %

82-93

1:800

Average %

Range %

87-101

回收率：

The recovery of human Plg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	90	85-97
EDTA plasma (n=4)	112	105-115

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



ng/ml	OD1	OD2	Average
4000	0.108	0.105	0.106
2000	0.214	0.216	0.215
1000	0.346	0.341	0.343
500	0.763	0.758	0.760
250	1.328	1.321	1.324
125	2.083	1.995	2.039
62.5	2.360	2.451	2.406
0	3.016	2.891	2.953