Rat matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08008r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠基质金属蛋白酶9/明胶酶B(MMP-9/Gelatinase B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的MMP9含量。MMP9 即基质金属蛋白酶 9，是细胞外基质降解的关键酶。在生理上参与组织重塑等过程，病理上在肿瘤侵袭转移、炎症等疾病中异常表达。研究机制集中在其表达调控、活性调节及与其他分子相互作用等方面，是潜在治疗靶点。试剂盒检测范围为0.78 ng/ml-50 ng/ml.，灵敏度为0.195 ng/ml.。适用于心血管疾病模型、肿瘤微环境研究、慢性炎症性疾病等科研领域，能够稳定检测不同样本来源的MMP - 9动态变化，为体外实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Mmp9 ELISA Kit; Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
缩写：
MMP9
Uniprot No.：
P50282
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
0.78 ng/ml-50 ng/ml.
灵敏度：
0.195 ng/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-9/Gelatinase B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-92
1:2	Average %	93
	Range %	91-105
1:4	Average %	96
	Range %	92-101
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
50	2.531	2.634	2.583	2.477
25	2.019	2.067	2.043	1.937
12.5	1.501	1.558	1.529	1.424
6.25	1.146	1.100	1.123	1.017
3.12	0.640	0.621	0.630	0.525
1.56	0.290	0.273	0.281	0.176
0.78	0.187	0.184	0.185	0.080
0	0.107	0.105	0.106