首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肌红蛋白(MYO/MB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MB含量。人肌红蛋白（MYO/MB）是一种存在于肌肉细胞中的蛋白质，主要功能是储存和运输氧气。其研究机制涉及氧储存、转运、清除自由基和调节一氧化氮水平等，对心肌和骨骼肌细胞的氧代谢至关重要。试剂盒检测范围为1.56 ng/mL-100 ng/mL，灵敏度为0.39 ng/mL。适用于基础科研领域，如动物模型中肌肉损伤的动态评估、疾病机制研究中肌红蛋白表达水平的分析，或药物干预后肌肉修复效果的体外实验验证；可为心血管疾病、运动医学及肌肉相关病理研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MB ELISA Kit; MGC13548 ELISA Kit; MYG_HUMAN ELISA Kit; Myoglobin ELISA Kit
缩写：MB
Uniprot No.：P02144
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MYO/MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 92 | ||||
| Range % | 89-99 | |||||
| 1:20 | Average % | 87 | ||||
| Range % | 83-96 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:80 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human MYO/MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 97 | 90-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.1752.2112.1932.107 501.6131.6561.6351.549 251.0241.0041.0140.928 12.50.5710.5840.5780.492 6.250.3120.3080.3100.224 3.120.1880.1950.1920.106 1.560.1290.1250.1270.041 00.0850.0870.086 | ||||||
货期：3-5 working days
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