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Human Layilin(LAYN) ELISA kit

  • 中文名: 人排卵蛋白(LAYN)酶联免疫试剂盒
货号: IPD-EL012772HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人排卵蛋白(LAYN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的LAYN含量。人排卵蛋白(LAYN)是一种调节T细胞功能的关键基因,其高表达与多种癌症的预后不良相关,尤其是与胃癌和结肠癌的免疫浸润水平密切相关。LAYN可能通过调节肿瘤相关巨噬细胞、DC、T细胞耗竭和Treg等免疫细胞在肿瘤微环境中的功能,影响肿瘤的进展和预后。试剂盒检测范围为15.6 pg/mL-1000 pg/mL,灵敏度为3.9 pg/mL。适用于体外研究LAYN在生殖生理调控、排卵障碍模型构建、卵巢功能评估以及免疫细胞相互作用等领域的表达特征,也可用于筛选调控LAYN表达的生物活性物质或药物分子,为生殖医学和免疫学基础研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Layilin ELISA Kit; LAYN ELISA Kit; LAYN_HUMAN ELISA Kit; UNQ208/PRO234 ELISA Kit
  • 缩写:
    LAYN
  • Uniprot No.:
    Q6UX15
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human LAYN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    83


    Range %

    80-86


    1:2

    Average %

    92


    Range %

    89-96


    1:4

    Average %

    104


    Range %

    101-107


    1:8

    Average %

    89


    Range %

    85-93


  • 回收率:

    The recovery of human LAYN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    102

    99-106


    EDTA plasma (n=4)

    101

    96-106


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.321

    2.341

    2.331

    2.159


    500

    1.929

    1.902

    1.916

    1.744


    250

    1.395

    1.376

    1.386

    1.214


    125

    0.875

    0.841

    0.858

    0.686


    62.5

    0.574

    0.592

    0.583

    0.411


    31.25

    0.396

    0.387

    0.392

    0.220


    15.6

    0.287

    0.291

    0.289

    0.117


    0

    0.171

    0.173

    0.172




  • 货期:
    3-5 working days

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